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Pyrosequencing of bisulfite dealt with DNA. Here is the sequencing of an amplicon created by a traditional forward primer but a biotinylated reverse primer to PCR the gene of decision. The Pyrosequencer then analyses the sample by denaturing the DNA and incorporating one nucleotide at any given time to the combo according to a sequence offered by t

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quick and straightforward strategy to change unmethylated cytosines from a DNA sample into uracils although methylated cytosines stay unchanged. The modified DNA is perfect for PCR amplification for downstream analyses, like restriction endonuclease digestion, sequencing, and microarrays. As well as the tumor classification, DNA methylation knowle

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